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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
31/07/2018 |
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Actualizado : |
11/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DOSTER, E.; ROVIRA, P.J.; NOYES, N.R.; BURGESS, B. A.; YANG, X.; WEINROTH, M.D.; LAKIN, S.M.; DEAN, C.J.; LINKE, L.; MAGNUSON, R.; JONES, K.I.; BOUCHER, C.; RUIZ, J.; BELK, K.E.; MORLEY, P.S. |
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Afiliación : |
ENRIQUE DOSTER, Microbial Ecology Group, Colorado State University, USA. Department of Microbiology, Inmunology and Pathology, Colorado State University. USA.; PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Microbial Ecology Group, Colorado State University. Department of Microbiology, Imnunology and Pathology, Colorado State University, USA.; NOELLE R. NOYES., Microbial Ecology Group, Colorado State University. Department of Veterinary Population Medicine, University of Minnesota.; BRANDY A. BURGESS, Department of Population Health, University of Georgia, USA.; XIANG YANG, Microbial Ecology Group, Colorado State University. Department of Animal Sciences, Colorado State University, USA.; MARGARET D. WEINROTH, Microbial Ecology Group, Colorado State University. Department of Animal Sciences, Colorado State University, USA.; STEVEN M. LAKIN, Microbial Ecology Group, Colorado State University. Department of Microbiology, Immunology and Pathology, Colorado State University, USA.; CHRISTOPHER J. DEAN, Microbial Ecology Group, Colorado State University. Department of Microbiology, Immunology and Pathology, Colorado State University, USA.; LYNDSEY LINKE, Department of Clinical Sciences, Colorado State University, USA.; ROBERTA MAGNUSON, Department of Clinical Sciences, Colorado State University, USA.; KENNETH I. JONES, Department of Biochemistry and Molecular Genetics, University of Colorado, USA.; CHRISTINA BOUCHER, Department of Computer and Information Science and Engineering, University of Florida, USA.; JAMIE RUIZ, Department of Computer and Information Science and Engineering, University of Florida, USA.; KEITH E. BELK, Microbial Ecology Group, Colorado State University. Department of Microbiology, Imnunology and Pathology, Colorado State University, USA.; PAUL S. MORLEY, Microbial Ecology Group, Colorado State University, USA. Department of Microbiology, Inmunology and Pathology, Colorado State University. USA. |
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Título : |
Investigating effects of tulathromycin metaphylaxis on the fecal resistome and microbiome of commercial feedlot cattle early in the feeding period. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Frontier in Microbiology, 2018, 9:1715. |
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Páginas : |
14 p. |
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DOI : |
10.3389/fmicb.2018.01715 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 14 April 2018; Accepted: 09 July 2018; Published: 30 July 2018.
Open Access journal.
https://doi.org/10.3389/fmicb.2018.01715 |
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Contenido : |
The objective was to examine effects of treating commercial beef feedlot cattle with therapeutic doses of tulathromycin, a macrolide antimicrobial drug, on changes in the fecal resistome and microbiome using shotgun metagenomic sequencing. Two pens of cattle were used, with all cattle in one pen receiving metaphylaxis treatment (800 mg subcutaneous tulathromycin) at arrival to the feedlot, and all cattle in the other pen remaining unexposed to parenteral antibiotics throughout the study period. Fecal samples were collected from 15 selected cattle in each group just prior to treatment (Day 1), and again 11 days later (Day 11). Shotgun sequencing was performed on isolated metagenomic DNA, and reads were aligned to a resistance and a taxonomic database to identify alignments to antimicrobial resistance (AMR) gene accessions and microbiome content. Overall, we identified AMR genes accessions encompassing 9 classes of AMR drugs and encoding 24 unique AMR mechanisms. Statistical analysis was used to identify differences in the resistome and microbiome between the untreated and treated groups at both timepoints, as well as over time. Based on composition and ordination analyses, the resistome and microbiome were not significantly different between the two groups on Day 1 or on Day 11. However, both the resistome and microbiome changed significantly between these two sampling dates. These results indicate that the transition into the feedlot?and associated changes in diet, geography, conspecific exposure, and environment?may exert a greater influence over the fecal resistome and microbiome of feedlot cattle than common metaphylactic antimicrobial drug treatment. MenosThe objective was to examine effects of treating commercial beef feedlot cattle with therapeutic doses of tulathromycin, a macrolide antimicrobial drug, on changes in the fecal resistome and microbiome using shotgun metagenomic sequencing. Two pens of cattle were used, with all cattle in one pen receiving metaphylaxis treatment (800 mg subcutaneous tulathromycin) at arrival to the feedlot, and all cattle in the other pen remaining unexposed to parenteral antibiotics throughout the study period. Fecal samples were collected from 15 selected cattle in each group just prior to treatment (Day 1), and again 11 days later (Day 11). Shotgun sequencing was performed on isolated metagenomic DNA, and reads were aligned to a resistance and a taxonomic database to identify alignments to antimicrobial resistance (AMR) gene accessions and microbiome content. Overall, we identified AMR genes accessions encompassing 9 classes of AMR drugs and encoding 24 unique AMR mechanisms. Statistical analysis was used to identify differences in the resistome and microbiome between the untreated and treated groups at both timepoints, as well as over time. Based on composition and ordination analyses, the resistome and microbiome were not significantly different between the two groups on Day 1 or on Day 11. However, both the resistome and microbiome changed significantly between these two sampling dates. These results indicate that the transition into the feedlot?and associated changes in diet, geography... Presentar Todo |
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Palabras claves : |
METAGENOMICS; METAPHYLAXIS; MICROBIOME; RESISTOME; TULATHROMYCIN. |
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Thesagro : |
BOVINOS; FEEDLOT. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/10933/1/fmicb-09-01715.pdf
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Marc : |
LEADER 02931naa a2200409 a 4500
001 1058855
005 2019-10-11
008 2018 bl uuuu u00u1 u #d
024 7 $a10.3389/fmicb.2018.01715$2DOI
100 1 $aDOSTER, E.
245 $aInvestigating effects of tulathromycin metaphylaxis on the fecal resistome and microbiome of commercial feedlot cattle early in the feeding period.$h[electronic resource]
260 $c2018
300 $a14 p.
500 $aArticle history: Received: 14 April 2018; Accepted: 09 July 2018; Published: 30 July 2018. Open Access journal. https://doi.org/10.3389/fmicb.2018.01715
520 $aThe objective was to examine effects of treating commercial beef feedlot cattle with therapeutic doses of tulathromycin, a macrolide antimicrobial drug, on changes in the fecal resistome and microbiome using shotgun metagenomic sequencing. Two pens of cattle were used, with all cattle in one pen receiving metaphylaxis treatment (800 mg subcutaneous tulathromycin) at arrival to the feedlot, and all cattle in the other pen remaining unexposed to parenteral antibiotics throughout the study period. Fecal samples were collected from 15 selected cattle in each group just prior to treatment (Day 1), and again 11 days later (Day 11). Shotgun sequencing was performed on isolated metagenomic DNA, and reads were aligned to a resistance and a taxonomic database to identify alignments to antimicrobial resistance (AMR) gene accessions and microbiome content. Overall, we identified AMR genes accessions encompassing 9 classes of AMR drugs and encoding 24 unique AMR mechanisms. Statistical analysis was used to identify differences in the resistome and microbiome between the untreated and treated groups at both timepoints, as well as over time. Based on composition and ordination analyses, the resistome and microbiome were not significantly different between the two groups on Day 1 or on Day 11. However, both the resistome and microbiome changed significantly between these two sampling dates. These results indicate that the transition into the feedlot?and associated changes in diet, geography, conspecific exposure, and environment?may exert a greater influence over the fecal resistome and microbiome of feedlot cattle than common metaphylactic antimicrobial drug treatment.
650 $aBOVINOS
650 $aFEEDLOT
653 $aMETAGENOMICS
653 $aMETAPHYLAXIS
653 $aMICROBIOME
653 $aRESISTOME
653 $aTULATHROMYCIN
700 1 $aROVIRA, P.J.
700 1 $aNOYES, N.R.
700 1 $aBURGESS, B. A.
700 1 $aYANG, X.
700 1 $aWEINROTH, M.D.
700 1 $aLAKIN, S.M.
700 1 $aDEAN, C.J.
700 1 $aLINKE, L.
700 1 $aMAGNUSON, R.
700 1 $aJONES, K.I.
700 1 $aBOUCHER, C.
700 1 $aRUIZ, J.
700 1 $aBELK, K.E.
700 1 $aMORLEY, P.S.
773 $tFrontier in Microbiology, 2018, 9:1715.
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Registro original : |
INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
27/04/2021 |
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Actualizado : |
27/04/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
BRIANO-RODRÍGUEZ, C.; ROMERO, A.; LLAMBÍ, S.; BRANDA, A.; FEDERICI, M.; GIANNITTI, F.; CAFFARENA, D.; SCHILD, C.; CASAUX, M.L.; DUTRA, F. |
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Afiliación : |
CAROLINA BRIANO-RODRIGUEZ, DILAVE "Miguel C Rubino" (MGAP), Laboratorio Regional Este, Treinta y Tres, Uruguay; AGUSTÍN ROMERO, DILAVE "Miguel C Rubino" (MGAP), Laboratorio Regional Este, Treinta y Tres, Uruguay; SILVIA LLAMBÍ, Facultad de Veterinaria, Universidad de la República (UDELAR), Uruguay; ANDREA BRANDA SICA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO DUTRA QUINTELA, DILAVE "Miguel C Rubino" (MGAP), Laboratorio Regional Este, Treinta y Tres, Uruguay. |
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Título : |
Lethal and semi-lethal mutations in Holstein calves in Uruguay. [Mutações letais e semi-letais em bezerros da raça Holandesa no Uruguai.] |
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Complemento del título : |
Animal Production. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Ciencia Rural, 2021, Volume 51, Issue 7, Article number e20200734. OPEN ACCESS. Doi: https://doi.org/10.1590/0103-8478cr20200734 |
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ISSN : |
0103-8478; eISSN 1678-4596. |
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DOI : |
10.1590/0103-8478cr20200734 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: August 07, 2020; Accepted: November 23, 2020; Revised: February 23, 2021.
This research was funded by grant N-15156 of the uruguayan Instituto Nacional de Investigación Agropecuaria (INIA). |
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Contenido : |
ABSTRACT.
Genetic disorders in Holstein cattle are a health problem that has grown worldwide in recent years, compromising the sustainability of modern dairy production. In Uruguay, Holstein-based milk production is one of the most important sectors of the country?s economy, but high levels of inbreeding have decreased the breed?s fertility in recent decades. This study investigated the presence and diffusion of lethal and semi-lethal alleles causing embryo death, abortions, fetal malformations, and neonatal diseases in Holstein calves. Using the GeneSeek® Genomic Profiler? Bovine 50K BeadChip, we genotyped 383 calves (1?30 days-old) from 27 farms located in the main dairy region of Uruguay. Results showed a high prevalence of farms (85%) and carrier calves (21%), including one or more of the following semi-lethal or lethal alleles: Syndactylism (4.18%), brachyspina (3.39%), cholesterol deficiency haplotype (2.61%), complex vertebral malformation (2.09%), bovine leukocyte adhesion deficiency (1.04%s), and Holstein haplotypes HH1 (4.44%), HH3 (3.13%), HH4 (1.04%), and HH5 (0.26%). Most of these alleles had not been recognized previously in Uruguay. We concluded that lethal and semi-lethal mutations are widespread in the Holstein breed in Uruguay. More studies are required to determine their impact on dairy cattle fertility. © 2021, Universidade Federal de Santa Maria. All rights reserved.
RESUMO.
Os distúrbios genéticos nos bovinos da raça Holandesa são um problema de saúde que cresceu nos últimos anos a nível mundial, comprometendo a sustentabilidade da produção leiteira moderna. No Uruguai, a produção leiteira com base na raça Holstein é um dos setores mais importantes da economia do país, mas altos níveis de endogamia diminuíram a fertilidade da raça nas últimas décadas. O objetivo deste estudo foi investigar a presença e difusão de alelos letais e semi-letais causando morte de embriões, abortos, malformações fetais e doenças neonatais em bezerros da raça Holandesa. Usando o BeadChip Bovino 50K GeneSeek® Genomic Profiler?, genotipamos 383 bezerros (menos de um mês) de 27 fazendas localizadas na principal região leiteira do Uruguai. Os resultados mostraram uma alta prevalência de fazendas (85%) e bezerros portadores (21%), incluindo um ou mais dos seguintes alelos letais ou semi-letais: sindactilismo (4,18%), braquipespina (3,39%), haplótipo de deficiência de colesterol (2,61%), malformação vertebral complexa (2,09%), deficiência de adesão de leucócitos bovinos (1,04% s) e haplótipos de Holstein HH1 (4,44%), HH3 (3,13%), HH4 (1,04%) e HH5 (0,26%). A maioria desses alelos não havia sido reconhecida anteriormente no país. Concluímos que as mutações letais e semi-letais são comuns na raça Holstein no Uruguai. Mais estudos são necessários para determinar seu impacto na fertilidade do gado leiteiro. MenosABSTRACT.
Genetic disorders in Holstein cattle are a health problem that has grown worldwide in recent years, compromising the sustainability of modern dairy production. In Uruguay, Holstein-based milk production is one of the most important sectors of the country?s economy, but high levels of inbreeding have decreased the breed?s fertility in recent decades. This study investigated the presence and diffusion of lethal and semi-lethal alleles causing embryo death, abortions, fetal malformations, and neonatal diseases in Holstein calves. Using the GeneSeek® Genomic Profiler? Bovine 50K BeadChip, we genotyped 383 calves (1?30 days-old) from 27 farms located in the main dairy region of Uruguay. Results showed a high prevalence of farms (85%) and carrier calves (21%), including one or more of the following semi-lethal or lethal alleles: Syndactylism (4.18%), brachyspina (3.39%), cholesterol deficiency haplotype (2.61%), complex vertebral malformation (2.09%), bovine leukocyte adhesion deficiency (1.04%s), and Holstein haplotypes HH1 (4.44%), HH3 (3.13%), HH4 (1.04%), and HH5 (0.26%). Most of these alleles had not been recognized previously in Uruguay. We concluded that lethal and semi-lethal mutations are widespread in the Holstein breed in Uruguay. More studies are required to determine their impact on dairy cattle fertility. © 2021, Universidade Federal de Santa Maria. All rights reserved.
RESUMO.
Os distúrbios genéticos nos bovinos da raça Holandesa são um problema de saúde ... Presentar Todo |
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Palabras claves : |
BLAD; Bovine hereditary diseases; Brachyspina; Braquipespina; Cholesterol deficiency; Complex vertebral malf; Deficiência de colesterol; Doenças hereditárias; Haplótipos holandeses; Holstein haplotypes; Syndactyly. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
https://www.scielo.br/pdf/cr/v51n7/0103-8478-cr-51-07-e20200734.pdf
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Marc : |
LEADER 04383naa a2200397 a 4500
001 1061998
005 2021-04-27
008 2021 bl uuuu u00u1 u #d
022 $a0103-8478; eISSN 1678-4596.
024 7 $a10.1590/0103-8478cr20200734$2DOI
100 1 $aBRIANO-RODRÍGUEZ, C.
245 $aLethal and semi-lethal mutations in Holstein calves in Uruguay. [Mutações letais e semi-letais em bezerros da raça Holandesa no Uruguai.]$h[electronic resource]
260 $c2021
500 $aArticle history: Received: August 07, 2020; Accepted: November 23, 2020; Revised: February 23, 2021. This research was funded by grant N-15156 of the uruguayan Instituto Nacional de Investigación Agropecuaria (INIA).
520 $aABSTRACT. Genetic disorders in Holstein cattle are a health problem that has grown worldwide in recent years, compromising the sustainability of modern dairy production. In Uruguay, Holstein-based milk production is one of the most important sectors of the country?s economy, but high levels of inbreeding have decreased the breed?s fertility in recent decades. This study investigated the presence and diffusion of lethal and semi-lethal alleles causing embryo death, abortions, fetal malformations, and neonatal diseases in Holstein calves. Using the GeneSeek® Genomic Profiler? Bovine 50K BeadChip, we genotyped 383 calves (1?30 days-old) from 27 farms located in the main dairy region of Uruguay. Results showed a high prevalence of farms (85%) and carrier calves (21%), including one or more of the following semi-lethal or lethal alleles: Syndactylism (4.18%), brachyspina (3.39%), cholesterol deficiency haplotype (2.61%), complex vertebral malformation (2.09%), bovine leukocyte adhesion deficiency (1.04%s), and Holstein haplotypes HH1 (4.44%), HH3 (3.13%), HH4 (1.04%), and HH5 (0.26%). Most of these alleles had not been recognized previously in Uruguay. We concluded that lethal and semi-lethal mutations are widespread in the Holstein breed in Uruguay. More studies are required to determine their impact on dairy cattle fertility. © 2021, Universidade Federal de Santa Maria. All rights reserved. RESUMO. Os distúrbios genéticos nos bovinos da raça Holandesa são um problema de saúde que cresceu nos últimos anos a nível mundial, comprometendo a sustentabilidade da produção leiteira moderna. No Uruguai, a produção leiteira com base na raça Holstein é um dos setores mais importantes da economia do país, mas altos níveis de endogamia diminuíram a fertilidade da raça nas últimas décadas. O objetivo deste estudo foi investigar a presença e difusão de alelos letais e semi-letais causando morte de embriões, abortos, malformações fetais e doenças neonatais em bezerros da raça Holandesa. Usando o BeadChip Bovino 50K GeneSeek® Genomic Profiler?, genotipamos 383 bezerros (menos de um mês) de 27 fazendas localizadas na principal região leiteira do Uruguai. Os resultados mostraram uma alta prevalência de fazendas (85%) e bezerros portadores (21%), incluindo um ou mais dos seguintes alelos letais ou semi-letais: sindactilismo (4,18%), braquipespina (3,39%), haplótipo de deficiência de colesterol (2,61%), malformação vertebral complexa (2,09%), deficiência de adesão de leucócitos bovinos (1,04% s) e haplótipos de Holstein HH1 (4,44%), HH3 (3,13%), HH4 (1,04%) e HH5 (0,26%). A maioria desses alelos não havia sido reconhecida anteriormente no país. Concluímos que as mutações letais e semi-letais são comuns na raça Holstein no Uruguai. Mais estudos são necessários para determinar seu impacto na fertilidade do gado leiteiro.
653 $aBLAD
653 $aBovine hereditary diseases
653 $aBrachyspina
653 $aBraquipespina
653 $aCholesterol deficiency
653 $aComplex vertebral malf
653 $aDeficiência de colesterol
653 $aDoenças hereditárias
653 $aHaplótipos holandeses
653 $aHolstein haplotypes
653 $aSyndactyly
700 1 $aROMERO, A.
700 1 $aLLAMBÍ, S.
700 1 $aBRANDA, A.
700 1 $aFEDERICI, M.
700 1 $aGIANNITTI, F.
700 1 $aCAFFARENA, D.
700 1 $aSCHILD, C.
700 1 $aCASAUX, M.L.
700 1 $aDUTRA, F.
773 $tCiencia Rural, 2021, Volume 51, Issue 7, Article number e20200734. OPEN ACCESS. Doi: https://doi.org/10.1590/0103-8478cr20200734
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